ECOPHYSIOLOGICAL ANALISIS SOME ASPECTS FOR THE REZISTING AND VIABILITY OF MOUSE EMBRYOS FREEZING

ECOPHYSIOLOGICAL ANALISIS SOME ASPECTS FOR THE REZISTING AND VIABILITY OF MOUSE EMBRYOS FREEZING

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Title:	ECOPHYSIOLOGICAL ANALISIS SOME ASPECTS FOR THE REZISTING AND VIABILITY OF MOUSE EMBRYOS FREEZING
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Article_Title:	ECOPHYSIOLOGICAL ANALISIS SOME ASPECTS FOR THE REZISTING AND VIABILITY OF MOUSE EMBRYOS FREEZING
Authors:	Svetlana KOGĂLNICEANU-ODOBESCU
Affiliation:	“Vasile Goldiş” Western University of Arad, Faculty of Life Sciences, Romania
Abstract:	The aim of this experimental work was to determine if the cryopreservation of mouse blastocysts affect their viability. We user 32 inception, 38 medium and 48 advanced blastocysts. Embryos were obtained by flushing the uterine horns of mouse females in day 4 p.c. (post coitus). "Cryocell-1200" was utilized for cryopreservation of embryos taking in to account data from literature. Cryopreservation doesn’t affect significantly the viability of preimplantation mouse embryos.
Keywords:	Fertilization, embryoculture, viability, cryopreservation, blastocyst, preimplantation mouse embryos
References:	Checiu M., Şandor Ş., – Rev. foum. Morphol. Embriol. Phisiol., Morphol-Embriol., 1982, 28, 127-133.♪ Cseh S., Wang G., Corselli J., Nehlsen-Cannarella S.L., Bailey L.L., Szalay A.A. – The effect of quickfreezinf in ethylene glycol on morphologikal survival and in vitro development of mouse embryos frozen at different preimplantation stages. Theriogenology vol. 47, nr.1, p. 342,1997.♪ Grigorescu Sido F., – Embriologie generală şi specială, Casa Cărţii de Ştiinţă, Cluj-Napoca, 1998.♪ Svetlana Kogâlniceanu-Odobescu – Possibilities of growing and handling of mouse embryos. Phd thesis, Timişoara, 1998.♪ Longo, F.J. – Fertilization, Chapman and Hall co., London-New York, 1987.♪ Muntean, M. I. – Groza-elements of Physiology of breeding animals, ed. Genessis, Cluj-Napoca, 1998.♪ Willis, M.W. – Atlas of descriptive embriology, Macmillan Publishing Co., New York, Collier Macmillan Publishers, London, 1982 Abbasali Karimpor Malekshah, Ph.D., Amir Esmailnejad Moghaddam – The effect of culture medium volume on in vitro development of mouse embryos, Iranian Journal of Reproductive Medicine Vol.3. No.2 pp:79-82, 2005
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Article Title:	ECOPHYSIOLOGICAL ANALISIS SOME ASPECTS FOR THE REZISTING AND VIABILITY OF MOUSE EMBRYOS FREEZING
Authors:	Svetlana KOGĂLNICEANU-ODOBESCU
Affiliation:	“Vasile Goldiş” Western University of Arad, Faculty of Life Sciences, Romania
Abstract:	The aim of this experimental work was to determine if the cryopreservation of mouse blastocysts affect their viability. We user 32 inception, 38 medium and 48 advanced blastocysts. Embryos were obtained by flushing the uterine horns of mouse females in day 4 p.c. (post coitus). "Cryocell-1200" was utilized for cryopreservation of embryos taking in to account data from literature. Cryopreservation doesn’t affect significantly the viability of preimplantation mouse embryos.
Keywords:	Fertilization, embryoculture, viability, cryopreservation, blastocyst, preimplantation mouse embryos
References:	Checiu M., Şandor Ş., – Rev. foum. Morphol. Embriol. Phisiol., Morphol-Embriol., 1982, 28, 127-133.♪ Cseh S., Wang G., Corselli J., Nehlsen-Cannarella S.L., Bailey L.L., Szalay A.A. – The effect of quickfreezinf in ethylene glycol on morphologikal survival and in vitro development of mouse embryos frozen at different preimplantation stages. Theriogenology vol. 47, nr.1, p. 342,1997.♪ Grigorescu Sido F., – Embriologie generală şi specială, Casa Cărţii de Ştiinţă, Cluj-Napoca, 1998.♪ Svetlana Kogâlniceanu-Odobescu – Possibilities of growing and handling of mouse embryos. Phd thesis, Timişoara, 1998.♪ Longo, F.J. – Fertilization, Chapman and Hall co., London-New York, 1987.♪ Muntean, M. I. – Groza-elements of Physiology of breeding animals, ed. Genessis, Cluj-Napoca, 1998.♪ Willis, M.W. – Atlas of descriptive embriology, Macmillan Publishing Co., New York, Collier Macmillan Publishers, London, 1982 Abbasali Karimpor Malekshah, Ph.D., Amir Esmailnejad Moghaddam – The effect of culture medium volume on in vitro development of mouse embryos, Iranian Journal of Reproductive Medicine Vol.3. No.2 pp:79-82, 2005
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