Study of Some Polyphenols from 1:5 Tinctures of Eryngium sp. from Romania by a HPLC-MS Method
April 1, 2010
| Abstract Title: | Study of Some Polyphenols from 1:5 Tinctures of Eryngium sp. from Romania by a HPLC-MS Method |
| Authors: | Suciu Simona*, Vlase Laurian** |
| Affiliation: | *Department of Clinical Pharmacy, “Vasile Goldis” Western University of Arad, Romania **Department of Pharmaceutical Technology and Biopharmaceutics, University of Medicine and Pharmacy “Iuliu Hatieganu”, Cluj-Napoca, Romania |
| Abstract text: | Among the 200 Eryngium species, only three species vegetate in the wild flora of Romania: Eryngium planum (plain eryngo), Eryngium campestre (fied eryngo) and Eryngium maritimum (sea holly). Since Antiquity, Eryngium species were known and recommended as remedies in the empiric medicine practice in a wide range of diseases for: the expectorant, diuretic, anti-inflammatory, aphrodisiac and anti-scorbutic properties. Drug Eryngii planii herba, representing the aerial parts collected during flourishing from Eryngium planum is an old well-known Romanian remedy, firstly introduced in phytotherapy in 1933 by Pater Bella. He drew attention upon the therapeutic potential of this drug, while visiting the Trascau Mountains where this drug was used as a very efficient remedy for children with whooping-cough. The other two indigenous species Eryngium , E. campestre and E. maritimum are relatively little use in the folk medicine of Romanian even though in the traditional folk practice of some Western Europe countries they were mentioned as: anti-inflammatory, diuretic, antiepileptic, expectorant and aphrodisiac. In modern phytotherapy these two species Eryngium campestre and Eryngium maritimum are strictly recommended for diuretic properties as cure for renal disorders (cystitis, nephritis, chronic prostatitis and for preventing kidney stones. The aim of the present study was to investigate the polyphenols (flavonoids and polyphenol acids) from the 1:5 tinctures of three indigenous Eryngium species. Polyphenols were determined both qualitatively and quantitatively by a standardized HPLC-MS method. Results of the 1:5 tinctures from the aerial parts of Eryngium planum, E. campestre and E. maritimum prepared in 70% ethanol were compared to the standards. Each sample was analyzed before and after hydrolysis in order to determine both glycosides and free aglycones, also. Results confirmed in the 1:5 tinctures obtained from the three Eryngium species from Romania the following compounds: isoquercitrin, quercitrin, rutosid, kaempferol, chlorogenic acid, p-coumaric acid and ferulic acid. |
| Keywords: | Eryngium, polyphenols, glycosides, flavonoids, phenolic acids |
| Presentation type: | Oral |
| Correspondence: | no. 17/10 Bd. Republicii, 17/10, Baia Mare, Maramures, Romania |
| Email: | suciu_simona@yahoo.com |
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