Effects of some plant extracts on HT-29 colorectal adenocarcinoma cell line
April 25, 2010
| Abstract Title: | Effects of some plant extracts on HT-29 colorectal adenocarcinoma cell line |
| Authors: | Maria Czapar1, George Pribac2, Regina Domina1, Vigh Szabolcs1, Endre Mathe1, Aurel Ardelean2, Violeta Turcus2, Simona Damian2, Liana Mos2, Coralia Cotoraci2 |
| Affiliation: | 1University College of Nyiregyhaza, Hungary 2“Vasile Goldis“ Western University of Arad, Romania |
| Abstract text: | From all the EU countries the greatest frequency of colorectal cancer is in Hungary (1.06‰) as well as the mortality caused by this disease (0.54‰). In this study we tested the in vitro activity of some plant extracts cultivated near the Romanian-Hungarian border that exert an antitumoral effect. For this study we have used colorectal adenocarcinoma cell line HT-29 (ATCC HTB-38) on McCoy’s culture medium 5A. In order to test the extracts we have used viability and proliferation tests MTT and Tunel assay. These tests highlight the apoptosis that takes place through DNA fragmentation. The fragmented DNA can be observed through enzymatic pathways, by biding the marked nucleotides (digoxigenin) on to the free ends of the DNA fragments. The extracts that were used were aqueous extracts of cherry (Prunus cerasus), black currant (Ribes nigrum) and beetroot (Beta vulgaris). For the statistical analysis we used the SPSS software (SPSS Inc. Headquarters, Chicago, Illinois, USA) and Mann-Whitney-U test with a statistic significance of p<0.05. Results show that the tested plant extracts testate have a biphasic effect on colorectal tumour cells HT-29; in high concentrations can inhibit and in low concentrations may stimulate the viability and proliferation of these cells (dose dependent effect). The extracts’ concentrations that exhibit a decrease of viability and proliferation is highest for beetroot extract (0.037%) and black currant extract (1.25%) compared to cherry extract that is reduced for the two concentrations used (2.5%). Tested extracts exert a significant effect on the viability and proliferation of tumoral cells. |
| Keywords: | plant extracts, viability, proliferation, HT-29 cells, colorectal adenocarcinoma |
| Presentation type: | Oral |
| Correspondence: | no. 1 Feleacului St., Arad, Romania |
| Email: | gpribac@uvvg.ro |
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