Flow cytometric analysis of encapsulated chondrocytes cultured in alginate gel for cartilage…

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Article Title:	Flow cytometric analysis of encapsulated chondrocytes cultured in alginate gel for cartilage tissue engineering
Authors:	Luminita Buia-Takacs1, Catalin Iordachel1, Ana-Maria Gheorghe1, Alexandrina Rugina1, Liana Mos2, Nicolae Efimov3, Daniela Bratosin1,4*
Affiliation:	1 National Institute of Biological Science Research and Development (INSB), Romania 2 ″Vasile Goldis″ Western University of Arad, Faculty of Medicine, Arad, Romania 3 Hospital CFR2, Bucharest, Romania 4 ”Vasile Goldis” Western University of Arad, Faculty of Biology, Arad, Romania
Abstract:	In autologous cell implantation (ACI), the autologous chondrocytes recovered from the patient are amplified in tissue culture prior to re-implantation. The ability to manipulate and reconstitute tissue structure and function in vitro has tremendous clinical implications and is likely to have a key role in cell therapies in coming years. The aim of this study was to investigate and to compare by flow cytometric methods, morphological changes, cellular viability and apoptosis of human chondrocytes cultured in alginate gel and conventional cell-seeding methods for cartilage tissue engineering applications. The results obtained provide the usefulness of the gel in the culture of chondrocytes for reconstructive clinical procedures.
Keywords:	chondrocytes, osteoarthritic cartilage, tissue engineering, flow cytometric analysis, microencapsulation, alginat gel, apoptosis, viability test
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*Correspondence:	Daniela Bratosin, National Institute for Biological Science Research and Development, Spl. Independentei nº296, 060031 Bucharest and ″Vasile Goldis″ Western University of Arad, Faculty of Natural Sciences, Arad, Romania, Tel/Fax: 40.21.2200881; E-mail: bratosind@yahoo.com